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fluoromount g  (SouthernBiotech)


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    Structured Review

    SouthernBiotech fluoromount g
    Fluoromount G, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 97/100, based on 10573 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoromount+g/Fluoromount-G/pm42929837-76-16-17
    Average 97 stars, based on 10573 article reviews
    fluoromount g - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    other:

    Article Title: CD47 fuels intestinal fibrosis by inducing fibroblast phagocytic evasion and represents a therapeutic target.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FACS-isolated mouse intestinal macrophages This paper Sorted from mouse colon single-cell suspensions for qPCR and SMART-seq/RNA-seq analyses Chemicals, peptides, and recombinant proteins Dextran sulfate sodium (DSS) MP Biomedicals Cat# 160110 2,4,6-trinitrobenzene sulfonic acid (TNBS) Meilunbio Cat# MB5523 Tamoxifen Sigma-Aldrich Cat# T5648 RNAlater Sigma-Aldrich Cat# R0901 Dulbecco’s Modified Eagle Medium (DMEM) Thermofisher Cat# 11960044 α-MEM KeyGEN BioTECH Cat# KGM11900-1 RPMI 1640 medium Thermofisher Cat# 22400089 Fetal bovine serum (FBS) Gibco Cat# 10091148 Penicillin/streptomycin KeyGEN BioTECH Cat# KGY0023 Murine M-CSF PeproTech Cat# 315-02-100 Recombinant TGF-β1 eBioscience Cat# 14-8342-80 Red blood cell lysis buffer Invitrogen Cat# 00-4300-54 Collagenase VIII Sigma-Aldrich Cat# C2139 DNase I Roche Cat# 5952077103 CFSE eBioscience Cat# 65-0850-84 Phosphate-buffered saline (PBS) Gibco Cat# 10010023 EDTA Gibco Cat# 15575020 Triton X-100 Beyotime Cat# P0096 DAPI (nuclear counterstain and flowcytometric viability dye) Sigma-Aldrich Cat# D9542 Fluoromount-G SouthernBiotech Cat# 0100-20 2× TransDirect Mouse Genotyping SuperMix TransGen Cat# AD501; used for mouse genotyping PCR 2× Phanta Flash Master Mix Vazyme Cat# P510; used for Col1a1-IRES-EGFP- IRES-MerCreMer mouse genotyping PCR MitoSOX Red Mitochondrial Superoxide Indicator Invitrogen/Thermo Fisher Scientific Cat# M36008 Critical commercial assays PanoPANEL kit Panovue Cat# 10234100050 Mouse IL-1β ELISA kit Sigma-Aldrich Cat# RAB0274 Mouse M-CSF/CSF1 ELISA kit Invitrogen/Thermo Fisher Scientific Cat# EMCSF1 Mouse TGF-β1 ELISA kit Invitrogen/Thermo Fisher Scientific Cat# BMS608-4 Deposited data Public human intestinal transcriptomic dataset GSE112366 NCBI Gene Expression Omnibus GEO: GSE112366 Public human intestinal transcriptomic dataset GSE16879 NCBI Gene Expression Omnibus GEO: GSE16879 Published human intestinal single-cell RNA-seq dataset Genome Sequence Archive GSA-Human: HRA006083; BioProject: PRJCA021346 Published mouse intestinal single-cell RNA-seq dataset Genome Sequence Archive GSA: CRA016292; BioProject: PRJCA021346 Published stricturing Crohn’s disease Visium spatial transcriptomic dataset Single Cell Portal Single Cell Portal: SCP2959 Published Crohn’s disease CosMx NanoString spatial dataset NCBI Gene Expression Omnibus GEO: GSE234713 (Continued on next page) e2 Cell Reports Medicine 7, 103103, October 20, 2026

    Fluorescence:

    Article Title: In Vivo Partial Cell Reprogramming With Yamanaka Factors Reduced Ischemic Brain Damage in Aging Mice
    Article Snippet: This was followed by three additional PBS washes, incubation with DAPI (1:5000; Millipore) for 10 min, and three final washes. .. Slides were mounted with Fluoromount‐G (SouthernBiotech) to preserve fluorescence. .. The following primary antibodies were used: mouse anti‐MAG (1:200; Millipore, MAB1567), rat anti‐MBP (1:300; Millipore, MAB386), rabbit anti‐PDGFRα (1:200; Abcam, ab20349), mouse anti‐BCAS1 (1:500; Santa Cruz, SC‐136342), mouse anti‐CC1 (1:200; Abcam, ab16794), rabbit anti‐Olig2 (1:200; Millipore, AB9610), rabbit anti‐NeuN (1:1000; Merck, ABN78), chicken anti‐GFAP (1:500; Abcam, ab4674), guinea pig anti‐Iba1 (1:750; lab clinics, HS‐234004), goat anti‐Klf4 (1:250; R&D system, af3158), and rabbit anti‐Oct4 (1:500; R&D system, SAB5700848).

    Article Title: Molecular Characterization of ALDH1A1 and Its Association with Secondary Hair Follicle Stem Cell Proliferation in Liaoning Cashmere Goats.
    Article Snippet: .. After washing, nuclei were counterstained with 4′,6-Diamidino-2-phenylindole (DAPI) (1:1000) for 5 min. Coverslips were mounted with Fluoromount-G (Southern Biotech, Birmingham, AL, USA) and examined using an Olympus IX73 fluorescence microscope (Olympus, Tokyo, Japan). ..

    Article Title: NRF2 activation attenuates obesity-induced adipose inflammation by suppressing STING signaling, with depot-specific regulation.
    Article Snippet: .. Samples were mounted with Fluoromount-G (SouthernBiotech, Cat. No. 0100-01) and imaged using a fluorescence microscope (DMi8, Leica) or a laser confocal microscope (C2, Nikon). .. Colocalization was quantified using ImageJ software (v1.53c) by calculating Pearson’s correlation coefficient.

    Microscopy:

    Article Title: Molecular Characterization of ALDH1A1 and Its Association with Secondary Hair Follicle Stem Cell Proliferation in Liaoning Cashmere Goats.
    Article Snippet: .. After washing, nuclei were counterstained with 4′,6-Diamidino-2-phenylindole (DAPI) (1:1000) for 5 min. Coverslips were mounted with Fluoromount-G (Southern Biotech, Birmingham, AL, USA) and examined using an Olympus IX73 fluorescence microscope (Olympus, Tokyo, Japan). ..

    Article Title: Sacubitrilat exerts potent antiarrhythmic effects through activation of natriuretic peptide receptor-C
    Article Snippet: .. Subsequently cells were washed three times in Blocking buffer and once in water before being mounted onto microscope slides with Fluoromount-G (Southern Biotech) and imaged with Thunder-DMi8 widefield microscope (Leica). ..

    Article Title: NRF2 activation attenuates obesity-induced adipose inflammation by suppressing STING signaling, with depot-specific regulation.
    Article Snippet: .. Samples were mounted with Fluoromount-G (SouthernBiotech, Cat. No. 0100-01) and imaged using a fluorescence microscope (DMi8, Leica) or a laser confocal microscope (C2, Nikon). .. Colocalization was quantified using ImageJ software (v1.53c) by calculating Pearson’s correlation coefficient.

    Blocking Assay:

    Article Title: Sacubitrilat exerts potent antiarrhythmic effects through activation of natriuretic peptide receptor-C
    Article Snippet: .. Subsequently cells were washed three times in Blocking buffer and once in water before being mounted onto microscope slides with Fluoromount-G (Southern Biotech) and imaged with Thunder-DMi8 widefield microscope (Leica). ..

    Widefield:

    Article Title: Sacubitrilat exerts potent antiarrhythmic effects through activation of natriuretic peptide receptor-C
    Article Snippet: .. Subsequently cells were washed three times in Blocking buffer and once in water before being mounted onto microscope slides with Fluoromount-G (Southern Biotech) and imaged with Thunder-DMi8 widefield microscope (Leica). ..

    Confocal:

    Article Title: NRF2 activation attenuates obesity-induced adipose inflammation by suppressing STING signaling, with depot-specific regulation.
    Article Snippet: .. Samples were mounted with Fluoromount-G (SouthernBiotech, Cat. No. 0100-01) and imaged using a fluorescence microscope (DMi8, Leica) or a laser confocal microscope (C2, Nikon). .. Colocalization was quantified using ImageJ software (v1.53c) by calculating Pearson’s correlation coefficient.

    Incubation:

    Article Title: Attenuation of Cardiac Dysfunction in the Collagen-Induced Arthritis Mouse Model Mediated by a Reactive Aldehyde Species Inhibitor.
    Article Snippet: Detection of anti-CIT antibody was carried out using AF488 AffiniPure Donkey anti-mouse IgM (1:1000; Jackson ImmunoResearch, West Grove, PA, USA). .. After a 45 min incubation with secondary antibody at room temperature, 4′,6-diamidino-2-phenylindole (DAPI) was applied, and samples were mounted with Fluoromount-G (Southern Biotech, Birmingham, https://doi.org/10.3390/ijms27198478 AL, USA). ..



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    ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were <t>counterstained</t> with <t>DAPI</t> (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.
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    ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were <t>counterstained</t> with <t>DAPI</t> (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.
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    Image Search Results


    ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were counterstained with DAPI (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.

    Journal: Advanced Science

    Article Title: Astrocyte‐Associated ABCA1/APOE Responses Accompany Hippocampal Lipid Remodeling and Ectopic Neuronal Accumulation During Epileptogenesis

    doi: 10.1002/advs.78329

    Figure Lengend Snippet: ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were counterstained with DAPI (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.

    Article Snippet: Cell nuclei were subsequently counterstained with DAPI (SBA‐0100‐20, 1:1000, SouthernBiotech).

    Techniques: Expressing, Western Blot, Analysis, Immunofluorescence, Marker, Multiplex Assay