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fluoromount g  (SouthernBiotech)


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    Structured Review

    SouthernBiotech fluoromount g
    Fluoromount G, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 97/100, based on 10451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoromount+g/Fluoromount-G/pmc12926594-128-14-17
    Average 97 stars, based on 10451 article reviews
    fluoromount g - by Bioz Stars, 2026-09
    97/100 stars

    Images

    Related Articles

    Laser-Scanning Microscopy:

    Article Title: RANKL-inducible Arl8b effector RUFY4 drives HOPS-mediated lysosomal maturation and trafficking in osteoclasts
    Article Snippet: Osteoclast-mediated bone resorption depends on the secretion of lysosomal hydrolases via the fusion of secretory lysosomes with the ruffled border, yet the molecular mechanisms governing lysosomal trafficking and fusion remain incompletely understood.. Here, we demonstrate that the small GTPase Arl8b regulates the processing of osteoclast-specific lysosomal hydrolase, cathepsin K, and the positioning of secretory lysosomes toward the actin ring.. Accordingly, depletion of Arl8b led to defects in lysosome-mediated bone resorption in osteoclasts.

    Staining:

    Article Title: RANKL-inducible Arl8b effector RUFY4 drives HOPS-mediated lysosomal maturation and trafficking in osteoclasts
    Article Snippet: Osteoclast-mediated bone resorption depends on the secretion of lysosomal hydrolases via the fusion of secretory lysosomes with the ruffled border, yet the molecular mechanisms governing lysosomal trafficking and fusion remain incompletely understood.. Here, we demonstrate that the small GTPase Arl8b regulates the processing of osteoclast-specific lysosomal hydrolase, cathepsin K, and the positioning of secretory lysosomes toward the actin ring.. Accordingly, depletion of Arl8b led to defects in lysosome-mediated bone resorption in osteoclasts.

    Incubation:

    Article Title: Retinoic acid regulates fetoplacental vascularization via notch signaling and a SEMA3E/F-PLEXIND1 axis
    Article Snippet: .. Sections were washed three times in 1X PBS +0.01% Triton X-100, then incubated with secondary antibodies in blocking solution for 1 h. After three additional washes in 1X PBS +0.01% Triton X-100, slides were mounted with glass coverslips using Fluoromount-G (Cat# 0100-01; SouthernBiotech, Birmingham, AL). ..

    Blocking Assay:

    Article Title: Retinoic acid regulates fetoplacental vascularization via notch signaling and a SEMA3E/F-PLEXIND1 axis
    Article Snippet: .. Sections were washed three times in 1X PBS +0.01% Triton X-100, then incubated with secondary antibodies in blocking solution for 1 h. After three additional washes in 1X PBS +0.01% Triton X-100, slides were mounted with glass coverslips using Fluoromount-G (Cat# 0100-01; SouthernBiotech, Birmingham, AL). ..



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    Image Search Results


    (A) Experimental paradigm for spontaneous withdrawal consisting of 7 days of twice daily escalating doses of morphine (or saline control), followed by a single final dose and 24 hours of abstinence before tissue collection. (B) Example images of anti-Fos immunostaining after spontaneous withdrawal paradigm, counterstained with DAPI. Dotted lines indicate boundaries of MHb and LHb. Arrow indicates Fos + cluster in the LHb. (C) Average distribution of Fos + cells across anterior-posterior axis of the habenula for each treatment condition. Contour lines indicate density of Fos + cells, normalized to the maximum density in the saline condition. (D) Quantification of the density of Fos + neurons in saline control and spontaneous withdrawal conditions. Each circle represents the mean for an individual animal. n=186 sections total from 10 mice per condition. **p< 0.01 by t-test. (E) Quantification of Fos + cells in all sections, binned every 400 μm along the A-P axis. Each circle represents one tissue section. (F) Same data in D, separated by sex. Two-Way ANOVA, treatment effect, **p< 0.01; sex effect, n.s.; interaction, n.s. Fisher’s post hoc, **p< 0.01. Open circles indicate female, filled circles indicate male.

    Journal: bioRxiv

    Article Title: Opioid withdrawal engages a habenular subpopulation responsive to aversive states

    doi: 10.64898/2026.04.29.721196

    Figure Lengend Snippet: (A) Experimental paradigm for spontaneous withdrawal consisting of 7 days of twice daily escalating doses of morphine (or saline control), followed by a single final dose and 24 hours of abstinence before tissue collection. (B) Example images of anti-Fos immunostaining after spontaneous withdrawal paradigm, counterstained with DAPI. Dotted lines indicate boundaries of MHb and LHb. Arrow indicates Fos + cluster in the LHb. (C) Average distribution of Fos + cells across anterior-posterior axis of the habenula for each treatment condition. Contour lines indicate density of Fos + cells, normalized to the maximum density in the saline condition. (D) Quantification of the density of Fos + neurons in saline control and spontaneous withdrawal conditions. Each circle represents the mean for an individual animal. n=186 sections total from 10 mice per condition. **p< 0.01 by t-test. (E) Quantification of Fos + cells in all sections, binned every 400 μm along the A-P axis. Each circle represents one tissue section. (F) Same data in D, separated by sex. Two-Way ANOVA, treatment effect, **p< 0.01; sex effect, n.s.; interaction, n.s. Fisher’s post hoc, **p< 0.01. Open circles indicate female, filled circles indicate male.

    Article Snippet: Sections were washed in 1xPBS and mounted on coverslips with Fluoromount-G with DAPI (Fisher Scientific, 50-112-8966).

    Techniques: Saline, Control, Immunostaining